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Effect <t>of</t> <t>endometriotic</t> cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in <t>KGN</t> granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.
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Effect <t>of</t> <t>endometriotic</t> cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in <t>KGN</t> granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.
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Effect <t>of</t> <t>endometriotic</t> cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in <t>KGN</t> granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.
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Informa UK Limited human granulosa like tumor cell line
Effect <t>of</t> <t>endometriotic</t> cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in <t>KGN</t> granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.
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Effect of endometriotic cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in KGN granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.

Journal: Biomedicines

Article Title: Peritoneal Endometriosis Impairs Ovarian Reserve and Increases Atresia in a Rat Model

doi: 10.3390/biomedicines13020348

Figure Lengend Snippet: Effect of endometriotic cells on the expression of folliculogenesis-related genes in granulosa cells in vitro. The AMH ( A ), AMHR2 ( B ), KITLG ( C ), and MTOR ( D ) mRNA expressions were assessed in KGN granulosa cells by RT-qPCR. KGN cells were cultured alone (KGN) or co-cultured with 12Z endometriotic cells (KGN + 12Z) for 48 h. 18S or RPLP0 were used as housekeeping genes. Results are expressed as means ± SEM. n is expressed in parentheses in each bar. Statistical comparisons were performed by Student’s “ t ” test. * p < 0.05 KGN vs. KGN + 12Z.

Article Snippet: In this study, we used the immortalized human epithelial-like endometriotic cell line 12Z [ ] and the human granulosa tumor cell line KGN (RIKEN Bioresource Centre, Tsukuba, Japan).

Techniques: Expressing, In Vitro, Quantitative RT-PCR, Cell Culture